the mir-383-ldha axis regulates cell proliferation, invasion and glycolysis in hepatocellular cancer
نویسندگان
چکیده
objective(s): to explore the correlation between expression patterns and functions of mir-383 and ldha in hepatocellular cancer (hcc). materials and methods: we detected the expression of mir-383 and ldha in 30 hcc tissues and their matched adjacent normal tissues using qrt-pcr. then we performed mtt assay, foci formation assay, transwell migration assay, glucose uptake assay and lactate production assay to explore the function of mir-383 in cell proliferation, invasion and glycolysis in hcc cell lines. luciferase reporter assay was used to explore whether ldha was a target gene of mir-383. western blot and qrt-pcr were used to further confirm ldha was targeted by mir-383. then the above functional experiments were repeated to see whether the function of ldha could be inhibited by mir-383. results: the results of qrt-pcr showed that mir-383 was down-regulated in hcc tissues compared with their matched adjacent normal tissues. functional experiments showed that overexpression of mir-383 significantly suppressed cell proliferation, invasion and glycolysis. luciferase reporter assay showed ldha was a target gene of mir-383 and expression of ldha was inversely correlated with that of mir-383 in hcc. besides, increased cell proliferation, invasion and glycolysis triggered by ldha could be inhibited by overexpression of mir-383 in hcc cell lines. conclusion: our study proved that mir-383 is down-regulated in hcc and acts as a tumor suppressor through targeting ldha. targeting the mir-383-ldha axis might be a promising strategy in hcc treatment.
منابع مشابه
The miR-383-LDHA axis regulates cell proliferation, invasion and glycolysis in hepatocellular cancer
Objective(s): To explore the correlation between expression patterns and functions of miR-383 and LDHA in hepatocellular cancer (HCC). Materials and Methods: We detected the expression of miR-383 and LDHA in 30 HCC tissues and their matched adjacent normal tissues using qRT-PCR. Then we performed MTT assay, foci formation assay, transwell migration assay, glucose uptake assay and lactate produc...
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عنوان ژورنال:
iranian journal of basic medical sciencesجلد ۲۰، شماره ۲، صفحات ۱۸۷-۱۹۲
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